The purification and enrichment of specific cell populations from complex starting samples is a critical component of multiple life science workflows. While the key objective of most protocols is to obtain a highly pure and viable population of cells for downstream applications and analysis, most do not meet this goal.
Issues with conventional cell separation methods, such as traditional magnetic beads and fluorescent activated cell sorting, include low recovery and poor cell viability, high costs and lengthy protocol times. Magnetic beads can remain associated with the isolated cells, causing decreased cell viability and changes to cellular function, and may interfere with downstream post-processing assays. In the quest to deliver new cellbased therapeutics, it is necessary to be able to efficiently scale up cell separation workflows to process higher volumes of samples that meet emerging regulatory requirements.